Microbial Analysis of Soil Essay Example for Free

Microbial Analysis of Soil Essay Abstract: soil samples were collected fortnightly from area near Dahisar River, A river in suburb of Mumbai. laboratory analysis started from July 2010 to September 2010. Total bacterial and fungal count were estimated by standard spread plate isolation. Isolated bacteria were subject to colony characterization and were estimated by their morphological and biochemical characters. As being a monsoon the occurrence of variation of different species were high. The microorganisms isolated from the soil were of staphylococcus strain and were gram positive, aerobic, coccus shaped bacteria. The fungal species were also identified, of which Aspergillus and Penicillium were dominant, followed by mucur, as sub dominant .This project aims to find out the water and soil quality of River and as it is flowing through an industrial area, to find out if it is getting affected by the Industrial pollutants. Introduction: Soil is the region on the earth’s crust where geology and biology meet, the land surface that provides a home to plant animal and microbial life (Pelczar et al., 1993). Soil teems with microscopic life (bacteria, fungi, algae, protozoa and viruses) as well as macroscopic life such as earthworms, nematodes, mites, and insects, and also the root systems of plants. The numbers and kinds of micro- organisms present in soil depend on many environmental factors: amount and type of nutrients available, available moisture, degree of aeration, pH, temperature etc (Prescott et al., 1999). Soil bacteria and fungi play pivotal roles in various biochemical cycles and are responsible for the recycling of organic compounds (Wall and Virginia, 1999). Soil microorganisms also influence above- ground ecosystems by contributing to plant nutrition, plant health, soil structure and soil fertility (O’Donnell et al., 2001). Soil is generally a favorable habitat for the proliferation of microo rganisms, with micro colonies, developing around soil particles. Numbers of micro organism . In soil habitats normally are much higher than those in fresh water or marine habitats (Atals and Bartha, 1998). Bacteria make up the most abundant group of micro- organisms in the soil (3.0 x 106 – 5.0 x 108) per gram of soil, followed by the actinomycetes (1.0 x 106 – 2.0 x 107), fungi (5.0 x 103 – 9.0 x 106), yeast (I.0 x 103 – 1.0 x 106), algae and protozoa (1.0 x 103- 5.0 x 105) and nematodes (50 – 200) counts per gram of soil are wide differences in the relative proportions of individual bacteria genera found in particular soils (Atals and Bartha, 1998). Soil fungi may occur as free-living organisms or in mycorrhizal association with plant roots. Fungi are found primarily in the top 10 cm of the soil and are rarely found below 30 cm. They are most abundant in well-aerated and acidic soils (Domsch et al., 1980). Most fungi in soil are opportunistic (zymogenous). They grow and carry out active metabolism when conditions are favorable which implies adequate moisture, adequate aeration and relatively high concentrations of utilizable substrates (Postage, 1994; Miyanoto et al., 2002). In this research we isolate culturable heterotrophic bacteria and fungi from different top soil samples MATERIALS AND METHODS Laboratory analysis Preparation of materials The materials needed for this experiment include; glass wares (conical flasks, bijou bottles, pipettes, petri-dishes) and they were washed with detergents. These glass wares were rinsed thoroughly with clean distilled portable water and left to air dry before sterilizing them in the autoclave at 15ââ€" ¦C for 1 hour. Also, the laboratory cabinets on which the work would be carried out was swabbed with cotton wool soaked in methylated spirit to sterilize it before any microbiological analysis was carried out to avoid the growth and isolation of other organisms not present in the samples. After sterilization, the plates were allowed to cool to about 45 degrees before they were used. Microbiological evaluation Ten (10) grams of the soil sample for microbiological evaluation was weighed into 9ml of sterile water. Preparation of serial dilution goes thus: 1ml of the original stocks solution was poured into 9ml sterile distilled water and mixed thoroughly to give 10-2 of the original sample and this was done for each sample and the bottles labeled according to date of collection Isolation and Enumeration of Micro-organisms. 1gram of the samples was homogenized in 9mls of distilled water to obtain a ratio of 1:9 and the second diluted of each sample was plated using the pour plate technique. Sterile molten nutrient agar (NA), potato dextrose agar (PDA), macconky’s agar,(MA) manitol salt agar (MSA) and deoxycholate astrate agar (DCA) were used{the potato dextrose agar (PDA) was acidified). These agars were then added and left to solidify undisturbed. These plates were incubated 37oC for 24hours (incubation was aerobic) and the procedure was repeated using 10-2 finally the number of colonies per plates were counted and recorded. The acidified PDA was incubated at 25C for 3-7 days for microbial growth. Total Bacterial counts (Cfu/g) The total bacteria count for each sample was determined with the pour plate techniques using nutrient agar. The plates were incubated between 24hours at 370C and all colonies appearing on the end of the incubation period were counted using digital unlimited colony counter and the counts were expressed in colony forming unit per gram {CFU/g} of the sample. Colonies of bacteria developing on the plates were observed, isolated and reisolated on a fresh media until pure culture was obtained. Preparation of Pure Culture It is necessary to isolate organisms in pure culture before studying and identifying them because a pure culture originates from one cell. Characteristics colonies from the original culture on the plates were picked with a sterile wire loop (using surface streaking method) and this loop was used to make streak of the colony on the surface of newly prepared sterile agar plates of NA,MA MSA. These streak will space out the inoculants and discrete colony of a particular specie of organism and then incubated at 35-37oC for 24hours to enhance microbial growth. Distinct colonies were re-inoculated on another fresh agar plates in order to obtain a pure culture. The isolates were picked with sterile loop and streaked into prepared agar slants, labeled and incubated for growth after which they were kept in the refrigerator for future use and identification. Identification of Isolates These isolated bacteria were identified using both morphological culture characteristics (i.e. the color, shape, elevation, capacity, consistency, edge) and biochemical test (i.e. citrate, oxidase, indole, sugar fermentation, test etc.)and the bacteria were identified based on the results obtained from the above mentioned biochemical characterization results and the procedures include. Grams Staining Techniques A drop of distilled water was placed on a clean glass slide. The inoculating wire loop was sterilized by flaming until it was red hot (this is to prevent the invasion of unwanted micro- organisms that might be inhabiting the wire loop) in the blue flame of a Bunsen burner. The loop was allowed to cool and the small portion of each colony of microorganisms to be gram stained was picked and smeared in the drop of water (distilled) on the glass slide and then spread into a thin smear along the slide. The smear was air dried and passed through the blue flame. The smear was stained with 1%crystal violet and left for 1minutes (60secs) and then washed with running distilled water it was then stained again with Lugols iodine for another 60secs and also washed with running distilled water. The slide was decolorized rapidly with 75% alcohol in order to present the organism from having the color of the primary reagent and it was washed immediately with distilled water. The slide finally was flooded with a counter stain safranine (a secondary stain) for 60secons and also washed off with distilled water and allow to air dry. The slide was covered with a cover slide and observed under the microscope using oil immersion x 100 objective lens with immersion oil. The gram reaction of the isolated arrangement and the shape of the cell were observed and recorded. Gram positive (+ve) bacterial were characterized by a purple color (i.e. the primary stain) while the gram negative (-ve) bacteria were characterized by red color (i.e. the secondary stain) .This procedure is actually used to ascertain the component of each organisms cell wall. Motility Motility was determined by hanging drop techniques. Using loop, a little part of the colony of the organisms were grown in peptone water for 18hours and then placed in the grease free slide and covered with a Vaseline bound cover slip and then observed under x100 objective lens. A motile organism is then seen moving in the drop of liquid. Identification Of Mold Isolates Mold isolated was identified using cultural and morphological characteristics and according to (Fawole and Oso, 2001), microscopic observation was carried out using lacto phenol blue stain. Procedure for Mold Staining A drop of lacto phenol blue stain was dropped on a clean grease free sterilized glass slide and after this a sterile inoculating wire loop was used to pick the mycelium unto the glass slide from the mold culture .The mycelium was spread evenly on the slide. Teasing was carried out to separate the mycelium in order to get a homogenous mixture and the mixture was then covered with cover slips gently and then allowed to stay for some seconds before observing under x40 under the microscope. The microscope examination of actively growing mold was on the basis of structures bearing spores, presence or absence of septate. BIOCHEMICAL TESTS Catalase Test Catalase test demonstrates the presence of catalase enzyme by aerobic microorganisms. Catalase is an enzyme that catalysis the release of oxygen from hydrogen peroxide (H2O2). To test for catalase, a drop of 3% hydrogen peroxide solution was added to a slide and the organism to be tested for catalase production is brought in contact with the hydrogen peroxide. The production of gas bubbles however indicates a positive reaction and this shows that catalase enzyme is produced.(FawoleOso, 2001) Oxidase test This was carried out by placing a clean filter paper on the working bench or petri dishes and 2-3 drops of freshly prepared oxidase reagent was added to the isolate using a sterile inoculating wire loop. After this, a few quantity of oxidase reagent was added and a purple coloration was observed within 10-15minutes which indicated that the organisms is oxidase positive and according to Olutiola et al, 1991, a positive reaction is dependent on the presence of cytochrome. This test is also useful for the separation of Neisseria in mixed culture and in differentiating Pseudomonas from enteric bacteria. Indole test Olutiola et al, 1991, describes the test as one which is important in the differentiation of colonies and it depends on the production of indole from tryptophan by the organism. An inoculating loop was used to inoculate the organism into a test tube containing decarboxylase medium becomes violet. An uninoculated test tube serves as a control (i.e. remained yellow) Sugar fermentation test The ability of the isolates to utilize certain sugar as energy source was tested. If the organism does ferment a particular sugar, acid will be produced and gas may be produced or not. Acid production is indicated by color change of the medium from red to yellow and acid presence could also be detectable with a ph. indicator in the medium while the production of gas is indicated by a void produced in a Durham tube. The fermentation medium was prepared by 0.1g of sodium chloride and 0.1g of fermentable sugar (glucose) in 10ml of distilled water. An amount of 9ml of the medium was pipette into a test tube containing Durham’s tubes in replicates. 5ml of phenol red indicator was immediately discharged into the test tubes. The test tubes containing medium were sterilized in an autoclave at 121 o for 15minutes.After sterilization, each isolate were incubated in glucose Medium. An uninoculated test tube was also incubated for glucose to serve as a control. The test was also carried out using maltose, lactose, galactose, manitol, sucrose, fructose and mannose.(Olutiolaet al., 1991) Discussion: The abundance of bacteria and fungi in this study were typical of environment with high species richness and functional diversity. Despite the fact that it is possible that a number of bacteria and fungi may be missed in this study, the isolates could be readily assigned dominant (e.g. Bacillus sp, Aspergillus sp) or transient/succession roles in the isolation of organisms form different seasons, which form the basis of this study. In additions to the implications of the determination of the number of microorganisms during soil sampling, one should consider the qualitative aspect of the preservation of important species and groups of microorganisms and of the changes in these biochemical characteristics resulting from the variations in these counts. Although the results of this study would not be considered to be exhaustive, as it was done within the limits of facilities available in the laboratory, an insight into the population dynamics and distribution of culturable aerobic bacteria and fungi diversity has been elucidated. This is without prejudice to the possible influence which a substantial proportion of bacteria and fungi that are not culturable in vitro could have on the overall picture of event. It would require more modern technology (nuclei acid probes) to obtain such detailed overview of microbial diversity. This should be a subject of extension of this investigation in future. Conclusion Through this project, if emphasis is made on public health, the observation and findings show striking predominance of Salmonella typhi. And E.coli. E.coli being an enterobacter cause dysentery and S.typhi poses a great risk of typhoid. Health inspector and municipal authorities should look into this matter for further investigation and if possible improvement Acknowledgement Investigators are grateful to the Principal Management of S.V.K.M’s Mithibai College for constant encouragement support. And head of department of zoology Prof. V.V. Dalvie for providing me opportunities and Prof. Radhika D’souza, under whose guidance the project was successfully completed References 1 .Atals RM, Bartha R (1998). Microbial Ecology: Fundamentals and Applications. 4th Edition. Benjamin Cummings Publishing Company Inc. Addison Wesley Longman Inc. pp. 300 – 350. 2. Miyanoto T, Igaraslic T, Takahashi K (2002). Lignin–degradation ability of litter decomposing basidomycetes from picea forest of Hokkaida Myco.sci. (41): 105 – 110. 3. Domsch KH, Gaws W, Anderson TH (1980). Compendium of soil fungi 4. O’ Donnell AG, Seasman M, Macrae A, Waite I, Davies JT (2001). Plants and Fertilizers as drivers of change in microbial community structure and function in soil. Plant Soil (232): 135 – 145. 5. Pelczar MJ, Chan ECS, krieg NR (1993). Microbiology: Concept and Application International edition McGraw-Hill, USA. Pp 281-324. 6. Wall DH, Virginia RA (1999). Controls on soil biodiversity insights from extreme environments. Appl. Soil Ecol. (13): 137–150. 7. Fawole and Oso, 2001

How Does Culture Impact On International Business Management Essay

How Does Culture Impact On International Business Management Essay Introduction The study of (Pothukuchi, Damanpour, Choi, Chen, Park, 2002) examines the effect of dimensions of national and organizational culture differences on the performance of international joint venture (IJV). They used the data from a survey of executives from joint ventures between Indian partners and partners from other counties to do the research. Finally, Pothukuchi, Damanpour, Chen, Park (2002) found that there is a negative effect from culture distance on the performance of international joint venture originates from differences in national culture and differences in organizational culture. Whats more, they found that the presumed negative effect from culture distance on the performance of international joint venture originates more from differences in organizational culture than from differences in national culture. Therefore, from the study of (Pothukuchi, Damanpour, Choi, Chen, Park, 2002), we can know that differences in national culture have a negative impact on the performance of international joint venture. Moreover, differences in organizational culture also have a negative impact on the performance of international joint venture. Now, we know that differences in national culture and differences in organizational culture both have a significant impact on the performance of the international business. Differences in culture between partners have a significant impact on the international business. Therefore, it is very important and necessary for the international business people to study and understand how culture impact on the international business. Moreover, Sirmon and Lane (2004) said that when the domain of a social group is closer to the value-creating activities of an alliance, cultural differences between the partners members of that social group will be more disruptive. From Sirmon and Lane (2004), we can know that when cultural differences between the partners are very relevant to the value-creating activities of an alliance, cultural differences will significantly affect the performance of the international business. Therefore, it is very important and worthy to study how the cultural differences between the partners impact the performance of international alliance. Whats more, Sirmon and Lane (2004) found that when the domain of a social group is closer to the value-creating activities of an alliance, organizational culture differences between the partners members of that social group will be more disruptive than national culture differences. Furthermore, they also found that professional culture is the most relevant t o the value-creating activities of an alliance. Therefore, when the domain of a social group is closer to the value-creating activities of an alliance, the professional culture differences between the partners members of that social group will be the most disruptive. From the study of (Pothukuchi, Damanpour, Choi, Chen, Park, 2002) and the study of (Sirmon and Lane, 2004), the culture differences have a significant impact on the international business when the domain of a social group is closer to the value-creating activities of an alliance. This essay will discuss how the culture impact on the international business. Moreover, this essay will discuss how the culture differences impact the international business based on three main readings: (Morrison, 2006, p.168), the study of (Pothukuchi, Damanpour, Choi, Chen, Park, 2002) and the study of (Sirmon and Lane, 2004). Section 2 is definition of culture and international business. Section 3 is discussion and analysis of how the culture differences impact the international business. Section 4 is the conclusion and some suggestions. Section 2 Definition of Culture and International business Culture In order to discuss how the culture differences impact the international business, we must understand what culture is first. Culture can be broadly defined as , a learned, shared, compelling, interrelated set of symbols whose meanings provide a set of orientations for members of a society (Terpstra and David, 1991, p.6). From (Morrison, 2006, p.169), we can know that culture has many aspects, for example, value, behavior, beliefs, communication, sport, music and customs. International Business In order to discuss how the culture differences impact the international business, we should also understand what international business is. From (Morrison, 2006, p.5), we know that an international business forms when business activities of a company straddle two or more countries. A global business forms when a company expands to the extent that a large portion of its business is outside its home country (Morrison, 2006, p.6). Salk and Shenkar (2001, p.163) said that International alliances reside at the confluence of different cultures which include national, organizational and occupational cultures. Therefore, it is very important to study and understand how different cultures impact on the international business. Section 3 Discussion and analysis of how the culture differences impact the international business. Pothukuchi et al. (2002) found that partners cultural differences may have more influence on the performance of international alliance as those differences become more directly related to the alliances primary value-creating activities. An international alliances performance is driven by the alliances effectiveness in achieving its primary value-creating activities. Moreover, Harrison et al. (2001) said that resource complementarity between partners of international alliance is often a necessary condition to optimize this value creation. Therefore, in order to share, combine and leverage complementary resources, the partners employees must interact effectively. Many researchers found that national and organizational culture differences between these employees affect their interactions. This means that differences in national and organizational culture have a significant impact on the performance of international business. It is very important and necessary for the international busin ess people to study and understand different national cultures and organizational cultures. (Morrison, 2006, p.168) also said that culture differences can directly impact the success or failure of a project. He also said that achieving a successful outcome will depend on sensitivity to differences in cultures between partners. This means sensitivity to differences in norms of behavior, value systems and languages between the partners will impact the success or failure of a project. Whats more, Trompenaars (1994) also said that for international business, grasping the interacting cultural dynamic between the global and the local is the key to be successful. Therefore, for international business, sensitivity to differences in cultures and grasping the interacting cultural dynamic between the global and the local is very important because culture differences can directly affect the success or failure of a project. Moreover, Sirmon and Lane, (2004) said that cultural differences stems from national, organizational and professional cultures. The differences in national, organizational and professional culture can inhibit international alliance partners employees ability to interact effectively. This essay will discuss how the culture differences affect the international business from three different aspects: national culture, organizational culture, and professional culture. National Culture We know that different countries have different cultures. From (Morrison, 2006, p.172), nations are distinguishable from each other by a shared cultural history, for example, religion, language, or racial identity. Moreover, all these distinguishing characteristics blend into a national culture. Common language, shared religious, shared moral values, shared history, relationship between the individual and group, and attitudes to education are all the elements of national culture. National culture relates primarily to deep-seated values (Hofstede et al., 1990). National culture influences family life, organizational culture, education, and economic and political structures (Morrison, 2006, p.172). Sirmon and Lane, (2004) also said that the influence of national culture is strong and long lasting. Organizations, while they may develop their own specific values and behavior, are also highly influenced by the national culture of their home country (Morrison, 2006, p.202). Moreover, Hofst ede (1991) found that national culture explains 50% of the differences in managers attitudes, beliefs, and values. Albert (1991) said that national culture differences are clearly seen in economic and political systems, educational systems, and other institutions. Therefore, Sirmon and Lane, (2004) said that national culture differences between partners of the international alliance can challenge the development of successful relationships. Park and Ungson (1997) said that these challenges stem partially from the lack of shared norms or values. Moreover, this lack of common understanding may undermine the partners interpretation of each others strategic intent, which is crucial in global markets and partnerships (Hitt et al., 1995). Whats more, Hennart and Zengs (2002) research findings also suggests that differences in national culture can disrupt collaboration and learning between partners of the international alliance. Furthermore, Sirmon and Lane, (2004) said that a lack of shar ed values and norms may reduce effective communication, trust and knowledge sharing in joint ventures. They said that all these problems have been found to lead to lower the performance of the international business. However, differences in national culture can be beneficial. For example, when the managers work with the foreign partners, they will spend more effort on avoiding misunderstandings in international alliances than they would in domestic alliances. In this case, differences in national culture can lead to high-level communication and a more sustained collaboration. Therefore, differences in national culture also have a good and positive impact on the international business. Whats more, Chui et al. (2002) and Gibson (1999) also said that National culture has been shown to impact on major business activities, from capital structure to group performance. Now, we know that differences in national culture have a significant impact on the international business. Therefore, it is very important to study and understand how the national culture impact on the international business. This essay will also discuss how the national culture impact on the international business by discussing and analyzing how language and religions impact on the international business. (1). Languages Common language is an element of national culture. Language is the basic means of communication between people. Language facilitates social interaction and fosters a system of shared values and norms (Morrison, 2006, p.174). If partners of international alliance cannot have common language to communicate, they cannot cooperate and interact because they cannot communicate. Business people cannot do business with the people who speak different languages. Nowadays, the importance of English as a global language extends far beyond the number of native speakers. English as a global language and common language helps people who speak different languages to communicate. English language is an intercultural means of communicating. Business people can use English in their international business activities. Business people now can do business with the people who speak different languages by using English. English helps the business people overcome the problems of intercultural communication in their international business activities. Therefore, having a common language is a very important necessary condition for international business. Today, most of South America is Spanish-speaking, moreover, in recent years Spanish companies have expanded in the region, attracted by a perceived affinity with these markets derived from a common language(Morrison, 2006, p.175-176). Moreover, in a low-context culture, communication is clear and direct, such as America. In a high-context culture, much goes unsaid because ambiguity is the norm and directness is avoided, such as Asian cultures (Morrison, 2006, p.174-175). Difference in language culture will also impact international alliance partners employees communication and inhibit their ability to interact effectively. Therefore, knowing and understanding the partners language culture well is also very important for international business. (2). Religions The system of values and beliefs that characterizes a culture may be embodied in a particular religion (Morrison, 2006, p.179). As well as religious power, religion may exercise considerable political power. It can form a major unifying force in society. In countries where religion is a major element of the cultural environment, sensitivity to local religious beliefs and practices is particularly important in building business relations (Morrison, 2006, p.180). Research has identified over 15,000 distinct religions and religious movements among the worlds population (Barrett, 1997). Therefore, there are many people who have religious beliefs in this world. Knowing the local religious beliefs and the business partners religious beliefs well is very important for the international business. Moreover, many social associations and political parties in many countries are commonly based on religious affiliations. Moreover, these form an essential dimension of the business environment. For example, the growth in membership and influence of large churches in parts of the US impacts on the social and cultural environment of the communities in which they are located. If there is an established religion in a location, a business must take account of its wide ramifications. If there are multiple religions in locations, a business as a good corporate citizen should not discriminate (Morrison, 2006, p.181-182). Knowing the business partners religious beliefs well will help to achieve good outcomes. Do not know the business partners religious beliefs well may lead to the failure of your business. Therefore, we can know that it is particularly important and necessary for the international business to know the business partners local religious beliefs well. Whats more, many aspects of business life are directly (or indirectly) affected by religious beliefs and practices. For example, particular foods that are forbidden such as beef for Hindus; ban or restrictions on consumption of alcoholic drink; religious festivals during which work may be forbidden; clothing requirements; requirement for women and men to be segregated in the work environment in Muslim societies; and the ban on Sunday shopping in some countries (Morrison, 2006, p.187). Organizational Culture From the study of (Pothukuchi, Damanpour, Choi, Chen, Park, 2002), we know that the negative effect on the performance of international joint venture originates more from differences in organizational culture than from differences in national culture. Whats more, from the study of (Sirmon and Lane, 2004), we know that when the domain of a social group is closer to the value-creating activities of an alliance, organizational culture differences between the partners members of that social group will be more disruptive than national culture differences. Therefore, from the study of (Pothukuchi, Damanpour, Choi, Chen, Park, 2002) and the study of (Sirmon and Lane, 2004), we can know that organization culture has more impact on the international business. Organizational culture is very important for the international business. Therefore, in order to discuss how the culture differences impact the international business, we must understand what organizational culture is. From (Morrison, 2006, p.195), organizational culture or corporate culture, like national culture, focuses on values, norms and behavioral patterns shared by the organization. An organizational culture is one that is deliberately fostered among employees, who may have come to the company from a variety of different cultural background. Characteristics of organizational culture include that, for example, common language, shared terminology, norms of behavior, and dominant values of the organization. Hofstede et al. (1990) said that whereas national culture relates primarily to deep-seated values, organizational culture relates primarily to shared beliefs in organizational practices and processes. Weber et al. (1996) found that different organizational cultures between acquirer and target could decrease top managers positive attitudes toward the merger and decrease their cooperation. Therefore, Sirmon and Lane, (2004) said that organizational culture is very important for the success of mergers and acquisitions. They thought that when the partners have the same organizational culture, satisfaction, learning and effectiveness of interactions will be increased. Therefore, same organizational culture between the partners has positive impact on the success of mergers and acquisitions. Whats more, they also thought that if the partners have different organizational cultures, satisfaction, learning and effectiveness of interactions will be decreased. Moreover, from (Sirmon and Lane, 2004), we know when learning, satisfaction and effectiveness of interactions are decreased, the business processes used to share combine and leverage resources will be inhibited. Sirmon and Lane, (2004) said that it will be more difficult for partners who have different organizational cultures to effectively achieve the primary value-creating activities. Professional culture From (Van Maanen and Barley, 1984; Jordan, 1990 ¼Ã¢â‚¬ ºBrown and Duguid, 1991; Lave and Wenger, 1991), we know that professional cultures develop through the socialization. Individuals receive the socialization during their training and occupational education. Then this socialization will be reinforced through the individuals professional experiences and interactions that lead to a broad understanding of how their occupation should be conducted. Moreover, Trice and Beyer (1993) said that professional cultures form as people, who span individual organizations, share a set of norms, values and beliefs related to their occupation. Sirmon and Lanes (2004) research findings suggests that professional culture differences are often the most relevant and salient cultural differences that the interacting employees face, and thus professional culture differences are the most disruptive to the alliances effectiveness in achieving its primary value-creating activities Section 4 Conculsion This essay discussed and analyzed that how the culture impact on the international business. Moreover, this essay discussed how the culture differences impact the international business based on three main readings: (Morrison, 2006, p.168), the study of (Pothukuchi, Damanpour, Choi, Chen, Park, 2002) and the study of (Sirmon and Lane, 2004). After discussing how the culture differences impact the international business, we know that: An international alliances performance is driven by the alliances effectiveness in achieving its primary value-creating activities. Moreover, resource complementarity between partners of international alliance is often a necessary condition to optimize this value creation. In order to share, combine and leverage complementary resources, the partners employees must interact effectively. However, national and organizational culture differences between these employees affect their interactions. Therefore, partners cultural differences may have more influence on the performance of international alliance as those differences become more directly related to the alliances primary value-creating activities. Achieving a successful outcome will depend on sensitivity to differences in cultures between partners. This means sensitivity to differences in norms of behavior, value systems and languages between the partners will impact the success or failure of a project. Therefore, culture differences can directly impact the success or failure of a project. Cultural differences stems from national, organizational and professional cultures. The differences in national, organizational and professional culture can inhibit international alliance partners employees ability to interact effectively. Differences in national culture lead to the lack of shared norms and value. The lack of shared values and norms may reduce effective communication, trust and knowledge sharing in the international business. Moreover, this lack of common understanding may undermine the partners interpretation of each others strategic intent, which is crucial in global markets and partnerships. All these problems have been found to lead to lower the performance of the international business. Therefore, differences in national culture can disrupt collaboration and learning between partners of the international alliance. Differences in national culture may have a negative impact on the performance of the international business. In some circumstance, differences in national culture can lead to high-level communication and a more sustained collaboration between the partners. Differences in national culture also have a good and positive impact on the international business. Therefore, differences in national culture can also be beneficial for the international business. Knowing and understanding the partners language culture well is also very important for international business. In countries where religion is a major element of the cultural environment, sensitivity to local religious beliefs and practices is particularly important in building business relations. When the domain of a social group is closer to the value-creating activities of an alliance, organizational culture differences between the partners members of that social group will be more disruptive than national culture differences. Therefore, the negative effect on the performance of international joint venture originates more from differences in organizational culture than from differences in national culture. Moreover, different organizational cultures between acquirer and target could decrease top managers positive attitudes toward the merger and decrease their cooperation. Organizational culture is very important for the success of mergers and acquisitions. Professional cultures form as people, who span individual organizations, share a set of norms, values and beliefs related to their occupation. Professional culture differences are often the most relevant and salient cultural differences that the interacting employees face, and thus professional culture differences are the most disruptive to the alliances effectiveness in achieving its primary value-creating activities. Some suggestion for the international business which reside at the confluence of different cultures: Morrison, (2006, p.202) said that in the international environment, a polycentric approach will make it easier for the international organization to adapt to the different cultural environments of foreign operations. (Morrison, 2006, p.168) also said that joint ventures is a good way to make international business relationships successful. Whats more, cultures are not fixed and static. Cultures can change. Therefore, organizations should be capable of change as they expand internationally because there are interactions between cultures and growth of international markets and global brands. Albert, M. 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Comparing Pride in A Good Man is Hard to Find, Good Country People and

Pride in A Good Man is Hard to Find, Good Country People and Revelation    Pride is a very relevant issue in almost everyone's lives. Only when a person is forced to face his pride can he begin to overcome it. Through the similar themes of her short stories, Flannery O'Connor attempts to make her characters realize their pride and overcome it. In "A Good Man is Hard to Find," the grandmother is a typical Southern lady. This constant effort to present herself a Southern lady is where her pride is grounded. She criticizes the mother's traveling outfit, but she herself is wearing a prim and proper-and probably uncomfortable-outfit so that "anyone seeing her dead on the highway would know at once that she was a lady" (O'Connor, "A Good Man" 382). She recalls exactly how to find a certain plantation she used to visit, and the children convince their father to turn the car around. However, the grandmother realizes that the plantation is in another state but is too prideful to admit so. This pride follows her to the point of grace when The Misfit forces her to see reality. According to Ellen Douglas, the "evil in human hearts, and the possibility of grace, the gift of love, are made terrifyingly and magnificently real" when the grandmother, at gunpoint, admits that The Misfit really is, in her standards, a good man at heart (381). He is better able to express his beliefs about religion, but she has no firm foundation. When he says, "She would [have] been a good woman, if there had been somebody there to shoot her every minute of her life," he is revealing the fact that her pride, instead of her faith, has carried her through life (O'Connor, "A Good Man" 392). She has merely acted out the life of a typical Southern lady of he... ...e. Grace allows this change to happen, but one must be willing to face hardships and difficulties because the road to redemption is narrow and rocky. Works Cited Douglas, Ellen. "O'Connor's 'A Good Man is Hard to Find.'" Contemporary Literature Criticism. Eds. Carolyn Riley and Phyllis Carmel Mendelson. Detroit, MI: Gale, 1976. Vol. 6. 381. Edwards, Jr., Bruce L. "O'Connor's 'Good Country People.'" Masterplots II-Short Story Series.   Ed. Frank N. Magill. Pasedena, CA: Salem P, 1986. Vol. 2. 901-902. O'Connor, Flannery. "Good Country People." Meyer 392-406. --------. "A Good Man is Hard to Find." Meyer 381-392. --------. "Revelation" Meyer 407-420. Meyer, Michael, ed. The Bedford Introduction to Literature: Reading, Thinking, Writing. 5th ed. Boston: Bedford/St. Martin's, 1999. --------. "O'Connor on Theme and Symbol" Meyer 423-424. Comparing Pride in A Good Man is Hard to Find, Good Country People and Pride in A Good Man is Hard to Find, Good Country People and Revelation    Pride is a very relevant issue in almost everyone's lives. Only when a person is forced to face his pride can he begin to overcome it. Through the similar themes of her short stories, Flannery O'Connor attempts to make her characters realize their pride and overcome it. In "A Good Man is Hard to Find," the grandmother is a typical Southern lady. This constant effort to present herself a Southern lady is where her pride is grounded. She criticizes the mother's traveling outfit, but she herself is wearing a prim and proper-and probably uncomfortable-outfit so that "anyone seeing her dead on the highway would know at once that she was a lady" (O'Connor, "A Good Man" 382). She recalls exactly how to find a certain plantation she used to visit, and the children convince their father to turn the car around. However, the grandmother realizes that the plantation is in another state but is too prideful to admit so. This pride follows her to the point of grace when The Misfit forces her to see reality. According to Ellen Douglas, the "evil in human hearts, and the possibility of grace, the gift of love, are made terrifyingly and magnificently real" when the grandmother, at gunpoint, admits that The Misfit really is, in her standards, a good man at heart (381). He is better able to express his beliefs about religion, but she has no firm foundation. When he says, "She would [have] been a good woman, if there had been somebody there to shoot her every minute of her life," he is revealing the fact that her pride, instead of her faith, has carried her through life (O'Connor, "A Good Man" 392). She has merely acted out the life of a typical Southern lady of he... ...e. Grace allows this change to happen, but one must be willing to face hardships and difficulties because the road to redemption is narrow and rocky. Works Cited Douglas, Ellen. "O'Connor's 'A Good Man is Hard to Find.'" Contemporary Literature Criticism. Eds. Carolyn Riley and Phyllis Carmel Mendelson. Detroit, MI: Gale, 1976. Vol. 6. 381. Edwards, Jr., Bruce L. "O'Connor's 'Good Country People.'" Masterplots II-Short Story Series.   Ed. Frank N. Magill. Pasedena, CA: Salem P, 1986. Vol. 2. 901-902. O'Connor, Flannery. "Good Country People." Meyer 392-406. --------. "A Good Man is Hard to Find." Meyer 381-392. --------. "Revelation" Meyer 407-420. Meyer, Michael, ed. The Bedford Introduction to Literature: Reading, Thinking, Writing. 5th ed. Boston: Bedford/St. Martin's, 1999. --------. "O'Connor on Theme and Symbol" Meyer 423-424.

Virginia: State and National Science Standards :: Essays Papers

Virginia: State and National Science Standards Across the field of education, standards have become both a guideline for teacher lesson plans and a measuring device for students’ achievement. In recent years several subject-based standards have been put in place by national organizations of scientists, mathematicians, and other professionals. Simultaneously, states have been developing their own set of standards, many of which may or may not correspond with offerings such as the National Science Education Standards (NSES). One example we will focus on in this paper is the State of Virginia’s Standards of Learning (SOLs). As a result, the VA SOLs received a rather poor grade on a national scale, measured by the Thomas B. Ford Foundation in March 1998. Much of the criticism lies in the VA SOLs’ lack of broad ideas and theoretical structure. Educators across the state have differing opinions concerning the VA Science Standards. Many educators have expressed disagreement over the value and the implementation of the SOLs. With regard to the VA Science SOLs, one elementary school teacher stated, "I don’t find the science SOLs controversial at all. I really enjoy teaching science and can find many ways to include the SOLs in all my academic areas." Not all teachers feel so positively about the standards. A Northern Virginia elementary principal expressed this viewpoint when he said, "Your feeling about the science SOLs will depend on your philosophical point of view about a fact vs. experiential science curriculum†¦Teachers who like open-ended, experiential-thinking based curriculum will not like them [VA SOLs], teachers who like sequential, fact-rich curriculum will." One criticism of the VA SOLs points towards its lack of exploratory learning due to its fact-based nature. Many believe that the extensive content needed to be covered handicaps teachers in such a way that they are unable to teach anything else but the SOLs and therefore can not delve deeply into any one area of study. On the contrary, VA SOLs can be a valuable resource for lesson plans. Since all teachers on a certain grade level must hold their students responsible for the same information, they can easily share lessons and materials. This lends itself to collaborative teaching. The NSES also have both positive and negative aspects. The National Science Education Standards appeal to those who appreciate an inquiry-based, constructivist approach. As stated in Catherine Kelly’s article, Reaching to the Standards (2000), "The Standards [NSES] have also encouraged constructivism and rallied support for a developmental curriculum in which students are more process oriented than product oriented.

Physiological Adaptations of the Plesiosaur for ?Holding its Breath? :: essays research papers

Physiological Adaptations of the Plesiosaur for ‘Holding its Breath’ One hundred and fifty million years ago, large aquatic species of reptile such as the Plesiosaur dominated the ocean, and were pre-eminent predators of the sea. The branch of now extinct Plesiosaurs, or ‘near lizards’, evolved into variant closely related species specialised to take different niches in the food chain. Such species of Plesiosaur include the phenotypically similar Plesiosauroid and Pliosauroid. The physiological adaptations of the long necked variant, the Plesiosauroid, as it relates to deep sea diving, will be addressed in depth. Oxygen breathing lungs are a universal trait of class reptilia. As such, it would have been necessary for the Plesiosauroid - a marine reptile, to return to the ocean surface to inhale air. Oxygen expenditure in reptiles is proportional to strenuosity of locomotion (Frappell, Schultz & Christian, 2002). Therefore the Plesiosauroid must have held physiological traits that enabled the species to avoid oxygen deficit while hunting deep-sea dwelling prey. This essay will outline the hypothesised respiratory, circulatory, pulmonary and sensory attributes of the Plesiosauroid as they relate to diving. These hypotheses will be supported by investigating the physiological adaptations of the Plesiosaur’s biological analogues, and the prospect of similar adaptations in the former will be speculated upon. Reptiles have a low metabolic rate: they consume energy, and therefore oxygen, slowly. According to Robinson (1975), Plesiosauroids were enduring swimmers with lower flipper aspect ratios and drag-causing long necks. Massare (1988) made the same conclusion, since the hydrodynamic properties of the Plesiosauroids indicate the species moved no faster than 2.3 metres every second. Therefore, the species was confronted by a conundrum: it sought to dive hundreds of metres to hunt its prey yet was constrained, by virtue of its body shape, to travel at slow speed. Invariably, the animal would have been required to forgo oxygen for periods of more than a minute, while keeping the presence of mind to hunt. Fortunately, when making its descent of hundreds of metres, the Plesiosauroid would have been able to exploit traits possessed by many of the reptile class. Many reptiles hold the ability to temporarily slow their heart rate to reduce their oxygen consumption, via bradycardia. This effect may be caused by low temperatures, such as is found deeper in the ocean, or may be voluntarily triggered by the animal. There would be no need for the Plesiosauroid to retain all of its oxygen-consuming faculties during the long descent.

Importance of Books in Life

Learning is a significant process in human being life. It is self-evidence that human development can not stand without a good learning. To reach that target, people always search the appropriate ways. Besides, the mains important elements that help people to learn are books and experiences because of their unlimited benefits. The book is a valuable source of knowledge that consists of infinite benefits. It transports us into different worlds and cultures, as well as, it informs us about ancient civilizations and lore.In addition, it helps us to learn about new technologies and literature. It allows speaking languages fluently and to communicate spontaneously. Therefore, the book can be faithful friend and the nearest into us which can guide us to how to behave in our practical life. Our practical life is also, in addition to books, based on experience. This latter is a crucial element that assists to overcome obstacles. Experience keeps our minds aware of the way to solve some probl ems, for example, if someone re-confront a problem, it would be easy for him/her to solve it.Experience also could indicate to us the suitable method of living in our life. In conclusion, learning is a strong challenge that requires patience and efforts. Books and experiences are the best manners of learning because of their vast importance and great advantages. Books can play a very important role in shaping our life because we can learn lots of things easily from books. Books provide a great solution in front of us to learn about anything and increase our knowledge level.Therefore, large numbers of people around the world read books because they find them very useful. Books are present in our world from many centuries in different forms and shapes of books are changing with time. Today, we can find books in pdf or audio formats as per the need of people. Though, all these books provide same help by increasing our knowledge levels. People can easily buy books from various book stor es available in market or they can use online web stores to order bestselling books. Best Answer – Chosen by VotersThe importance of reading books is gaining new insight or perspectives on issues that matter to the reader that helps them to have a new understanding of the world around them. Readers can apply the insight in everyday life or as a way of building upon the knowledge that already encompasses their mind and enrich their lives. The importance of books are also found in how new discoveries pertaining to history are uncovered and/or revealed where the relevance of this the new information needs to be applied or amended in what already exists about a particular issue, topic or event.Thus, â€Å"new† books on existing issues are important because sometimes facts â€Å"change. † Books are a tangible, valuable record of our world. Sure we have the internet and computers with vast amounts of information stored in them but all of history isn't in a computer. H istory, the stories of civilations long gone and ancient languages forgotten are somewhere in a book. Books are there so you can learn, imagine and sometimes escape from the world around you. There is nothing like sitting down with a new book, the pages crisp and fresh, and reading about a love story, or a horrible murder.Books are an outlet for many people. For some it is their way of expressing their imagination†¦ writers create so we can read and let our minds take us to a different place, a different time, maybe even a different world. For others, books are an escape. When the world gets to be too crazy and you need to escape you can pick up a book and forget about the world around you. Books enables us to see life through other peoples eyes. People are able to communicate their view of the world to a large audience.Books also enables us to understand other cultures, which in turn enables us to relate better with others. Books can have a profound effect on society and the w orld because they enable an individual to change the world. A single book by a single author can make a huge difference in the whole world. Think ‘Tuesdays with Morrie', ‘God has a dream' and you begin to see the role of books in society and why they are important. Books glue people and cultures together. But unfortunately they can also be used to do just the opposite of the above.

Example of Bad Essay Writting

TREASURE HUNT On one fine day, me and my four other friends were planned to have sushi’s as our lunch at the famous Japanese restaurant that just opened recently. While heading to the restaurant, I’ve noticed that there is an old man having a difficulty collecting his stuffs that just fell on the street. Thus, we decided to help the old man collecting his stuffs back to his bag. While collecting his stuffs, we noticed that, there are lots of old things such as an old paper and many more. After we’ve finished picked up his stuffs and placed it properly in his bag, the old man thanks us for helping him picking up his stuffs.Then, the old man wants to thanks us by giving out one of his stuffs which old papers that looks just like a mystery map. The old man told us it is not an old piece of an ordinary paper, it is a lost treasure map that will guide us to find a treasure that has been buried in a jungle called Goggie jungle. At first we don’t believe wheatear the old man is telling us the truth or not. But after listened to his interesting story, we were really curious whether there is a treasure buried in the Goggie jungle or not. Thus we all have decided to have an expedition together to hunt the buried treasure hiding in the jungle.A week before we went to the jungle, we have planned on what to bring along and what not to. Thus we have listed out the reason why we chosen the items to bring along with us in our expedition. First must item that we have chosen to bring along are spade. The purpose of a spade is to help us dig out the buried treasure. Plus, it is also can be used as one of our self defend items that could help us kill or chase away wild animals that could be found in the jungle such as snakes or even wild lizards that could cause us to be in a danger while in the jungle.Since it is a very useful item, we decided to bring two spades along with us for the expedition. Secondly, we have chosen to bring a compass together wit h us. A compass is used to identified and guide us towards to the location of the buried treasure from the map. Thus, the main function of compass is to shows the direction that we should follow in order us to find the exact location in the jungle and this could also help us from getting lost in the jungle. Next, we have chosen to bring a rope along with us the expedition.The purpose of a rope is to help us cross the river that might be blocking us way towards the treasure location. Other than that, a rope is used to help us to go up and down the steep hill and helps us to tie the tent. The fourth item that we have chosen to bring along with us is torchlight. A torchlight is used as a light sources at night and it is also can be used as an S. O. S sign if we ever get lost in the jungle. In addition, torchlight can also be used as a self-defend item where, the light could help us chase wild animals that could be hiding behind the bushes at night.Furthermore, we have chosen to bring a knife together with us. The purpose of a knife is to cut off the bushes that might be preventing our way towards to our destination. It is also used as a self-defend if we are in a danger while in the jungle. Plus, a knife is used as a cooking utensil item where, we used a knife to cut off fruits or vegetables that we could find in the jungle. Last but not least, a compulsory item that we must bring along with us in the expedition is binocular.A binocular is to look out things that are far away from us. This could help us to stay alert and prevent us with things that might cause us to be in danger while in jungle. After finished choosing the important items that we should bring along with us in our expedition which is spades, rope, torchlight, knife, compass and a binocular. All these items that we have chosen are very useful and it could help us to be successful complete the treasure hunt. Thus, we are all excited to start our first experience of a treasure hunt.